Advanced Research Journal of Biotechnology. Vol. 1 (1), pp. 001-007, October, 2013.© Advanced Scholars Journals
 

Full Length Research Paper

Molecular characterization of staphylococcus aureus isolated from wounds

 Wurochekke A.U, Obanibi W.O and Sarkiyayi S

 Department of Biochemistry, Modibbo Adama University of Technology Yola, PMB 2076, Adamawa State. Nigeria.

*Corresponding author. E-mail: [email protected] 

 Accepted 21 October, 2013

 Abstract

 This study was carried out to determine the molecular characterization of staphylococcus aureus isolated from wound sources in ABU teaching hospital. 10 samples were collected from the hospital. They were sub cultured, the  morphological test, the microbiological test, the biochemical test and the molecular biology tests were carried out. The molecular techniques that require the isolation of genomic DNA, polymerase chain reaction (PCR) for the  amplification of a gene sequence were carried out. The PCR-machine used was 312-techgene, 9 samples were amplified during the reaction, which means 9 samples were positive. The s. aureus strain associated with the methicillin  resistance gene (femAgene) was amplified at 647bp. The specific gene for femA gene was amplified at 647bp. With the advantage of it being assayed for within 5 hours using molecular techniques compared to the conversational method  of testing which take up to 4 days. S. aureus was present on healthy humans of all ages, sex and is a multidrug resistance microorganism which can result to complicated infection, if not diagnosed and treated earlier. This molecular  technique should be employed in the hospital for early diagnosis and appropriate treatment to prevent complicated infections.

 Key words: Methicillin resistance gene ,wounds, isolation, Genomic  DNA, microorganism.