International Research Journal of Plant and Crop Sciences ISSN: 1711-3490 Vol. 3 (3), pp. 107-114, November, 2017.  © Advanced Scholars Journals

Full Length Research Paper 

Allelopathic potential of C. spinosum aqueous and organic extracts on the seed germination

*Issam Bouazizi Marzouki, Radhi Kechiche and Riadh Mebazaa

Laboratory of Genetic Biodiversity and Valorisation of Bioressources (LR11ES41), High Institute of Biotechnology, Tahar Haddad street, Monastir 5000, University of Monastir, Tunisia.

*Correspoonding Author. Email: [email protected]

Accepted 22 September, 2017

Abstract

Allelopathic potential of Citharexylum spinosum L. (Verbenaceae) an exotic tree introduced in Tunisia many years ago was evaluated. Organic extracts using hexane, ethyl acetate and methanol solvents together with aqueous extracts at different concentrations were prepared from different parts of the plant (roots, stems, leaves and flowers). Yields in the 12 organic extracts together with their phenol contents were reported. Leaves methanol extract showed the highest yield and amount in total phenols (6.43%; 617.93±1.12 mg gallic acid equivalent/100 g MS, respectively). All extracts were tested on germination and early growth of two crops: (Lactuca sativa L.) and (Triticum aestivum L.) and two weeds: (Peganum harmala L.) and (Silybum marianum L.). Twelve parameters were established and used to the principal components analysis (PCA) and the hierarchical clusters (HCA) analysis. Three groups of extracts were separated according to their allelopathic potentiality. Almost of organic extracts were totally opposed to seed germination of peganum and thistle.

Key words: Allelopathy, bioherbicides, Citharexylum spinosum L., crops, extracts, polyphenols, weed management.