Advanced Research Journal of Microbiology Vol. 1 (1), pp. 001-009, September, 2013. © Advanced Scholars Journals

Full length research paper

Identification and antimicrobial resistance profiles of Salmonellae isolated from the broiler dressing plants associated with their environments

Fahmitha Jahan, S. M. Lutful Kabir^* and M. Mansurul Amin

Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Accepted 8 September, 2012

*Corresponding author. E-mail: [email protected]. Tel: +88-091-67401-6  (ext.2394)

Abstract

The study was aimed at isolation and identification of Salmonella spp. from dressing water, device and environmental samples collected from pluck shops (cottage poultry processors) by using cultural characteristics, differential staining, biochemical techniques and motility test. Furthermore, the isolated Salmonella spp. was characterized by antimicrobial susceptibility testing. Among the 27 Salmonella   isolates, 22.22% (n=6) were identified as Salmonella pullorum (SP), 18.51% (n=5) as Salmonella gallinarum (SG), 59.26% (n=16) as Salmonella typhimurium (ST). Motility test indicated that 40.74% (n=11) isolates were non motile and 59.26% (n=16) were motile. As regards source of isolates, 33.33% (n=2) of SP was equally available in dressing water, device and environmental samples. On the other hand, 40% (n=2) of SG was detected in dressing water, 20% (n=1) in device and 40% (n=2) in environmental samples. However, 50% (n=8), 31.25% (n=5) and 18.75% (n=3) ST was detected respectively in dressing water, device and environmental samples. Antibiogram studies indicated that SP, SG and ST were more or less susceptible to chloramphenicol (C), azithromycin (AZ), ciprofloxaxin (CIP) and norfloxacin (NOR) but SP was also susceptible to gentamycin (CN). Besides being resistant to a number of antibiotics, the organisms revealed also multidrug sensitivity that calls attention of practitioner on the use of antibiotics.

Key words: Broiler dressing plant, salmonellae, identification, antimicrobial susceptibility.